修飾抗體 Products
酪氨酸硝基化 (Nitrotyrosine) 抗體
杭州景杰生物科技有限公司
Anti-nitro-Tyrosine mouse mAb

Cat#:

PTM-752

Ab Type:

M

Applications:

WB

Species:

All

Price: (100 μl)

2880.00

Price: (20 μl)

864.00


Product Description

The mouse-derived anti-3NT antibody is purified with Protein G. It specifically recognizes proteins with tyrosine nitrated residues but not the tyrosine phosphorylated or non-modified tyrosine residues. Besides common applications in Western blotting and immunoprecipitation, the antibody has been well utilized for proteomic screening to tyrosine nitrated substrates from protease-digested proteins both in prokaryotic and eukaryotic cells.


Source/Purification

This product is produced by immunizing mice with nitrated KLH. The ascites was purified by protein G.


Specificity

All nitrated proteins expected

Recommended Applications

ELISA, WB, IP???

*WB=Western blotting; ?IP=Immunoprecipitation


Recommended antibody dilution

WB: 1:1000

Important

For Western blotting, incubate membrane with diluted antibody in 5% BSA, 1 x TBS, 0.1% Tween-20 at 4℃ with gentle shaking, overnight.

Use at an assay dependent concentration. Optimal dilutions/concentrations should be determined by the end user. Not yet tested in other applications.

blob.png

Dot blotting?analysis on indicated amount of peptides using 3-nitrotyrosine mouse mAb. The list of peptides is included in the table on the right.?

blob.png

Western blot?analysis on 30 μg of extracts from HeLa and MEF whole cell lysates, untreated or treated with peroxynitrite(3 mM, 30 mins) or pervanadate(3 mM, 30 mins), using anti-3-nitrotyrosine mouse Antibody (Figure B) or anti-phospho-Tyrosine rabbit pAb (PTM-702) (Figure C).


Scientific Description

Nitrotyrosine is a product of tyrosine nitration mediated by reactive nitrogen species such as peroxynitrite anion and nitrogen dioxide. It is very stable and so is suitable for analysis in biological sample such as plasma, lung aspirants-BALF (Broncho alveolar lining fluid) and urine. Increased nitrotyrosine levels have been associated with several physiological and pathological conditions and is considered a marker of NO-dependent, reactive nitrogen species-induced nitrative stress. Research shows that nitrotyrosine levels can be reduced by N-acetyl cysteine which is a precursor to glutathione, one of the body's primary endogenous antioxidants.



Storage & Stability

The antibody is kept in PBS with 50% glycerol and 0.01% sodium azide. Upon receipt, please centrifuge the antibody at 12,000 x g for 20 seconds and?store the antibody at -20°C. Avoid repeated freeze/thaw cycles. Stable for 12 months from date of receipt. Leave the antibody at room temperature for 2 minutes and gently mixed using pipette before usage.?


**For research purposes only. Not intended to be used for therapeutic or diagnostic purposes in humans or animals.


ELISA, WB, IP???

*WB=Western blotting; ?IP=Immunoprecipitation


推薦產品 PRODUCTS
Copyright ?2018-2020 杭州景杰生物科技有限公司
犀牛云提供云計算服務
X
合作
交流
1

QQ設置

3

SKYPE 設置

4

阿里旺旺設置

等待加載動態數據...

等待加載動態數據...

5

電話號碼管理

  • 400-100-1145
6

二維碼管理

等待加載動態數據...

等待加載動態數據...

展開
合作交流
填寫信息,我們的專屬學術顧問將為您解答疑問!
上海十一选五走势图一定牛 什么叫胆码和拖码 11选5开奖 通用bbin下载app 配资盘股票配资网 上海快三专家预测推荐 产业基金配资是真的吗 上海11选5定胆 吉林快3个位走势图 银行怎么给私募基金配资 神算子精选心水资料 山东省群英会走势图 辽宁十一选五开奖结果手机版 香港免费一尾中特 重庆幸运农场平台 股票配资平台有哪些是正规的 2020生肖号码波色表图